Geddo F., Scandiffio R., Antoniotti S., Cottone E., Querio G., Maffei M. E. 2, Bovolin P. 1 and Gallo M. P.
\nPipeNig\u00ae-FL, a Fluid Extract of Black Pepper (Piper Nigrum L.) with a High Standardized Content of Trans-\u03b2-Cariofillene, Reduces Lipid Accumulation in 3T3-L1 Preadipocytes and Improves Glucose Uptake in C2C12 Myotubes.
\nNutrients 11.11 (2019): 2788.[\/vc_column_text][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#ffffff” section_id=”carattere” sidebar=”sidebar-1″][vc_column][mk_padding_divider size=”20″][mk_fancy_title color=”#2d2d2d” size=”28″ font_weight=”bold” margin_bottom=”0″ font_family=”none”]Bioactive Triterpenes of Protium heptaphyllum Gum Resin Extract Display Cholesterol-Lowering Potential[\/mk_fancy_title][mk_padding_divider size=”20″][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#ffffff” sidebar=”sidebar-1″][vc_column][vc_column_text el_class=”testo-nero” css=”.vc_custom_1651144838310{margin-bottom: 0px !important;}”]Hypercholesterolemia is one of the major causes of cardiovascular disease, the risk of which is further increased if other forms of dyslipidemia occur. Current therapeutic strategies include changes in lifestyle coupled with drug administration. Statins represent the most common therapeutic approach, but they may be insufficient due to the onset of resistance mechanisms and side effects. Consequently, patients with mild hypercholesterolemia prefer the use of food supplements since these are perceived to be safer. Here, we investigate the phytochemical profile and cholesterol-lowering potential of Protium heptaphyllum gum resin extract (PHE). Chemical characterization via HPLC-APCI-HRMS2 and GC-FID\/MS identified 13 compounds mainly belonging to ursane, oleanane, and tirucallane groups. It was initially evaluated the variation of gene expression, via realtime PCR, of genes involved in the metabolism (biosynthesis and catabolism) of cholesterol and lipid metabolism. Studies on human hepatocytes have revealed how PHE is able to reduce cholesterol production and regulate the expression of proteins involved in its metabolism. (HMGCR, PCSK9, LDLR, FXR, IDOL, and PPAR). Moreover, measuring the inhibitory activity of PHE against HMGR, moderate inhibition was recorded. Finally, molecular docking studies identified acidic tetra- and pentacyclic triterpenoids as the main compounds responsible for this action. These experimental evaluations were conducted by comparing the action of Protium extract with the metabolically active form of lovastatin. These experimental evaluations were conducted by comparing the action of Protium extract with the metabolically active form of lovastatin. However, the mechanism of action does not appear to be directly related to the inhibition of the enzyme HMGR with a statin-like mechanism since the Protium extract showed a moderate inhibition of the aforementioned enzyme (Fig.3). Finally, molecular docking studies have identified tetra and pentacyclic acid triterpenoids as the main responsible for this action. In conclusion, our study demonstrates how PHE may be a useful alternative to contrast hypercholesterolemia, highlighting its potential as a sustainable multitarget natural extract for the nutraceutical industry that is rapidly gaining acceptance as a source of health-promoting compounds.<\/p>\n
Mannino G., Iovino P., Lauria A., Genova T., Asteggiano A., Notarbartolo M., Porcu A., Serio G., Chinig\u00f2 G., Occhipinti A., Capuzzo A., Medana C., Munaron L., Gentile C. (2021)
\nBioactive Triterpenes of Protium heptaphyllum Gum Resin Extract Display Cholesterol-Lowering Potential
\nFormaInt. J. Mol. Sci. 2021, 22(5), 2664[\/vc_column_text][\/vc_column][\/mk_page_section]\n","protected":false},"excerpt":{"rendered":"
[mk_page_section bg_color=”#ffffff” section_id=”carattere” sidebar=”sidebar-1″][vc_column][mk_padding_divider size=”20″][mk_fancy_title color=”#2d2d2d” size=”28″ font_weight=”bold” margin_bottom=”0″ font_family=”none”]Quali\u2013Quantitative Characterization of Volatile and Non-Volatile Compounds in Protium heptaphyllum (Aubl.) Marchand Resin by GC\u2013MS Validated Method, GC\u2013FID and HPLC\u2013HRMS2[\/mk_fancy_title][mk_padding_divider size=”20″][\/vc_column][\/mk_page_section][mk_page_section bg_color=”#ffffff” sidebar=”sidebar-1″][vc_column][vc_column_text el_class=”testo-nero” css=”.vc_custom_1652691871767{margin-bottom: 0px !important;}”]Protium heptaphyllum (Aubl.) Marchand (PH) trees are endemic to the tropical region of South America, mostly Brazil. Antibacterial, antinociceptive, anti-inflammatory, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1093","page","type-page","status-publish","hentry"],"yoast_head":"\n